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bacterial nebnext rrna depletion probes  (New England Biolabs)


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    Structured Review

    New England Biolabs bacterial nebnext rrna depletion probes
    Bacterial Nebnext Rrna Depletion Probes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+rrna+depletion+bacteria/NEBNext+rRNA+Depletion+Kit+(Bacteria)+with+RNA+Sample+Purification+Beads/pmc13672349-305-12-13
    Average 95 stars, based on 77 article reviews
    bacterial nebnext rrna depletion probes - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title: Soil bacterium Massilia secretes metabolites that promote Leptospira growth
    Article Snippet: L. interrogans L495 cells cultured for 48 hours under each condition ( ) were harvested by centrifugation and total RNA was extracted from pellet using an RNA purification kit (Zymo Research Corp., Irvine, CA, USA). .. RNA-seq libraries were prepared using NEBNext rRNA Depletion (Bacteria) (New England Biolabs, Ipswich, MA), TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, Inc., San Diego, CA) and sequenced on an NovaSeq X (Illumina, Inc., San Diego, CA) to generate 151-bp paired-end reads. .. Protocol for rRNA removal using the NEBNext rRNA Depletion Kit (Bacteria), was from the “TruSeq Stranded Total RNA Reference Guide” (1000000040499 v00).

    Bacteria:

    Article Title: Soil bacterium Massilia secretes metabolites that promote Leptospira growth
    Article Snippet: L. interrogans L495 cells cultured for 48 hours under each condition ( ) were harvested by centrifugation and total RNA was extracted from pellet using an RNA purification kit (Zymo Research Corp., Irvine, CA, USA). .. RNA-seq libraries were prepared using NEBNext rRNA Depletion (Bacteria) (New England Biolabs, Ipswich, MA), TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, Inc., San Diego, CA) and sequenced on an NovaSeq X (Illumina, Inc., San Diego, CA) to generate 151-bp paired-end reads. .. Protocol for rRNA removal using the NEBNext rRNA Depletion Kit (Bacteria), was from the “TruSeq Stranded Total RNA Reference Guide” (1000000040499 v00).

    Article Title: Differential producibility analysis reveals drug-associated carbon and nitrogen metabolite expressions in Mycobacterium tuberculosis
    Article Snippet: .. Ribosomal RNA was removed from total RNA using NEBNext rRNA Depletion (bacteria) (NEB). .. NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) was used for sequencing library preparation as instruction, including RNA fragmentation, first and second-stand cDNA synthesis, adapter ligation, indexing, and library fragment PCR enrichment.

    Article Title: Bovine Colostrum-Derived Extracellular Vesicles May Accelerate the Growth of Akkermansia muciniphila by Regulating Energy Metabolism in Intestinal Anaerobic Coculture System
    Article Snippet: Library construction and sequencing were performed by Macrogen (Seoul, Korea). .. Libraries were prepared using the NEBNext rRNA Depletion (Bacteria) and TruSeq Stranded Total RNA Library Prep Gold Kit and sequenced on an Illumina NovaSeq platform. ..

    Article Title: Mild acidic stress enhances freeze-drying survival in Leuconostoc mesenteroides WiKim0121 by activating carbohydrate transport and metabolism.
    Article Snippet: The aim of the present study was to explore the effect of fermentation pH during the cultivation of Leuconostoc mesenteroides WiKim0121 on its viability after harvesting and survival rate after freeze-drying (FD).. The viability and survival rate of Leu. mesenteroides WiKim0121 differed with fermentation pH ranging from 5 to 7 in a 5 L fermenter.. The highest viable cells of 6.73 × 109 CFU/mL was observed with the pH 6 controlled culture, whereas the highest survival rate of 89 % after FD was observed with the pH 5 controlled culture.

    Article Title: Lynronne antimicrobial peptides with terminal D amino acid enantiomers synergize with bedaquiline against Mycobacterium tuberculosis
    Article Snippet: .. Ribosomal RNA was 543 removed from total RNA using NEBNext rRNA Depletion (bacteria) (NEB). .. NEBNext Ultra II 544 RNA Library Prep Kit for Illumina (NEB) was used for sequencing library preparation as per 545 manufacturer’s instruction, including RNA fragmentation, first and second-strand cDNA 546 synthesis, adapter ligation, indexing and library fragment PCR enrichment.

    Sequencing:

    Article Title: Mild acidic stress enhances freeze-drying survival in Leuconostoc mesenteroides WiKim0121 by activating carbohydrate transport and metabolism.
    Article Snippet: The aim of the present study was to explore the effect of fermentation pH during the cultivation of Leuconostoc mesenteroides WiKim0121 on its viability after harvesting and survival rate after freeze-drying (FD).. The viability and survival rate of Leu. mesenteroides WiKim0121 differed with fermentation pH ranging from 5 to 7 in a 5 L fermenter.. The highest viable cells of 6.73 × 109 CFU/mL was observed with the pH 6 controlled culture, whereas the highest survival rate of 89 % after FD was observed with the pH 5 controlled culture.

    other:

    Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes.
    Article Snippet: Transcriptomic analysis of vegetative, sporulating and germinating cells a. RNA extraction Pellets of vegetative cell cultures in early exponential (2 d subculture in broth) and stationary growth (5 d subculture in broth), as well as germinating endospores (16 h post-inoculation), were resuspended in RNAprotect Bacteria reagent (QIAGEN) prior to RNA isolation with the RNeasy Mini kit (QIAGEN) with enzymatic lysis and on-column DNase digestion.

    Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes
    Article Snippet: The peptides were loaded onto a 75 μm × 2 cm Acclaim PepMap 100 C18 trap (Thermo Fisher Scientific) in solvent A (0.1% (v/v) formic acid) at a flow rate of 2 μl/min and eluted at 0.3 μl/min in a 5 to 40% gradient of solvent A and solvent B (0.1% (v/v) formic acid in 80% (v/v) acetonitrile).



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